Selective Inhibition of Bacterial Enzymes by Free Fatty Acids

Selective inhibition of bacterial enzymes by free fatty acids.

Octanoic acid inhibits, in vitro, the bacterial enzymes glucose-6-phosphate dehydrogenase, phosphofructokinase, pyruvate kinase, fumarase, lactate dehydrogenase, and the malic enzyme of Arthrobacter crystallopoietes. The free fatty acid appears to act as an inhibitor of lipogenesis, although it does not affect the rate of gluconeogenesis. To demonstrate that this inhibition may be of physiologi...

Inhibition of gluconeogenic enzymes by free fatty acids and palmitoyl coenzyme A.

The effects of free fatty acids on rat liver phosphoenolpyruvate carboxykiuase (EC 4.1.1.32), pyruvate carboxylase (EC 6.4.1. I), and glucose 6-phosphatase (EC 3.1.3.9) were determined to evaluate the suggestion that free fatty acid inhibition of key glycolytic enzymes constitutes a specific inhibitory effect. Activities of ah of these gluconeogenie enzymes were found to be markedly inhibited b...

Inhibition of enzyme activities by free fatty acids.

The inhibition of several enzyme activities by free fatty acids has been examined. The inhibition of rat liver microsomal linolenate-activating enzyme activity by linolenate was found to be due to the inactivation of enzyme by substrate fatty acid. Stearoyl coenzyme A was also found to inhibit linolenate-activating enzyme. Likewise, microsomal glucose 6-phosphatase was inhibited by oleate; the ...

Inhibition of Thermophilic Anaerobic Digestion of Waste Food by Long Chain Fatty Acids and Propionate

Myocytes by Free Fatty Acids

Using rat ventricular cells, we studied the actions of free fatty acids and their ability to modulate the ATP-sensitive K' channel and to activate a new type of ATP-insensitive K' channel previously identified in rat atrial cells. Perfusion of the cytoplasmic face of the membrane with unsaturated fatty acids (10-50 ,M) such as arachidonic, linoleic, and eicosatrienoic acids inhibited the ATP-se...